Fig. 6

Spatial distributions of 3 FTx in the venom gland of A. lubricus. A On the left, the 3 FTx phylogeny with the bootstraps displayed at nodes and the cytotoxins and neurotoxins colored in blue and red, respectively. On the right, spatial distributions of 3 FTx as determined by MSI are shown as heat-maps across two near-serial sections from the same venom gland. Sections are positioned in mirrored orientation and heatmap color legend is shown below. Bottom right shows (from top to bottom) a schematic representation of the venom gland connected to the fang, the orientation of the sections used for the MSI, and the unstained sections used for MSI (bottom). The anterior region, which is near to the fang, is indicated with “A” and the posterior region, which is distant from the fang, is indicated with “P”. The scale bar represents a size of 500 μm. B Pairwise genetic distance of peptide and promoter sequences and Jaccard similarity of TFs correlated to the pairwise spatial correlations of the 3 FTx paralogs obtained in the MSI. Comparisons within neurotoxins, within cytotoxins, and across them are colored in red, blue, and purple, respectively