Fig. 5
From: Parent-of-origin regulation by maternal auts2 shapes neurodevelopment and behavior in fish
Gene expression in oocytes and one-cell stage RNA-seq analysis. a RNAscope (auts2a probe and negative control probe) on ovarian sections. RNAscope probe staining is displayed in red (auts2a) and green (hdac1 and msi2a) and DAPI staining in blue. Oo: ooplasm, Nu: nucleus. b Immunostaining (AUTS2 primary antibody and negative control without primary antibody) on ovarian section. Oo: ooplasm, Nu: nucleus. Antibody staining is displayed in red and DAPI staining in blue. c Volcano plot of dysregulated genes in 1-cell stage embryos following maternal auts2a loss-of-function. Wild-type was used as the control condition. Red dots correspond to msia2, hdac1, and rb1. ns: genes that are not significantly differentially expressed between WT and mutant