Fig. 2
Total RNA-seq defines convincing O-decay genes A DEGs (FC = MII versus GV stage oocytes, adjusted P < 0.01, |log2(FC)|< 1) detected using total RNA-seq. B Relative RNA copy number between samples in total RNA-seq, calculated using ERCC%. Three replicates were calculated, and error bars represent SEM. P-values were calculated using two-tailed Student’s t test: n.s. P ≥ 0.05, *P < 0.05, and **P < 0.01. C Scatter plot comparing the DEGs defined by Smart-seq2 with those defined by total RNA-seq in MII versus GV stage oocytes. D Line plot comparing the percentage change in poly(A) length (as shown in Fig S5 A) between O-decay genes (red line) and all genes (gray line). E Line plot comparing the percentage change in TE (as shown in Additional file 1: Fig. Fig S5B) between O-decay genes (red line) and all genes (gray line). F Line plot comparing the percentage change in protein intensity (as shown in Additional file 1: Fig. S5 C) between O-decay genes (red line) and all genes (gray line)