Fig. 4

Minigene splicing assay for IFI44L. A Structure of IFI44L gene and the surrounding area of rs1333973. Exons are depicted by the thick lines. This figure was obtained from the UCSC genome browser. B Isoforms of IFI44L. Isoform structures are depicted using GSDS 2.0 [23]. C Motif prediction for NM_006820.3 and XM_005270391.3 using HMMER software. MR_HSR1, G-alpha, AIG1, AAA-PrkA, and ATP_bind_1 domains/motifs were predicted in the CDS of NM_006820, while no domain/motif was predicted in the CDS of XM_005270391.3. D Boxplots displaying gene expressions in the 67 Japanese B cell lines. The expression levels of the entire IFI44L gene (left) and four isoforms are presented by genotypes (AA (n = 42), AT (n = 23), and TT (n = 2)). E Agarose gel electrophoresis of RT-PCR products expressed from IFI44L (exon 2 and exon 3) minigenes in HEK293 cells. M: 100 bp ladder marker. Lane 1: pSPL3 + IFI44L(IVS2 + 3 A); Lane 2: pSPL3 + IFI44L(IVS2 + 3 T); Lane 3: Negative control. Blue boxes: exon of pSPL3 plasmid; green boxes: exon 2 of IFI44L; orange boxes: exon 3 of IFI44L