Fig. 5

DNA demethylase DNG702 and methylase DRM2 role in CR and LR development. A and B Histograms showing the transcription dynamics of DNG702 (A) and DRM2 (B) during crown, lateral, and embryonic root initiation. Two biological replicates are indicated. Arrows indicate the transcription trend during initiation. C and D Point mutant of DNG702 (ta2) (C) and overexpression of DRM2 (D) both affect rice crown and lateral root initiation. Histograms show the statistics of crown and lateral root numbers. Two biological replicates for ta2 mutant and two independent transgenic lines for DRM2 overexpression are performed. Tested numbers for each sample are indicated. Double and single asterisks indicate significant differences, P-value < 0.01 and < 0.05 (Student’s t-test). ns, no significant difference. Bars = 0.5 cm and 1 cm for LR and CR, respectively