Fig. 4

Developmental trajectories of wheat early endosperm. A Visualization of embryo/endosperm cells via UMAP colored by development stages (left) and respective cell types (right). B Visualization of the development trajectory of embryo/endosperm cells colored by development stages (left) and respective cell types (right). C Expression of the top 3000 highly variable genes along pseudotime. D KEGG enrichment for differentially expressed genes. The enriched KEGG categories were determined using the one-sided version of Fisher’s exact test, followed by the Benjamin-Hochberg correction to obtain adjusted p values for multiple testing. E Heatmap of the expression of cluster 1 and cluster 2