Fig. 8
Inference of spatially resolved pro-generative communication in the mouse skeletal muscle injury. a The mouse scRNA-seq dataset of the skeletal muscles under the uninjured and post-injury day 2, 5, and 7 conditions induced by notexin, involving the uninjured myofiber, injured locus, and border. b Heatmap showing the number of MiTIs between pairwise cell types in uninjured and injured muscles as well as the miRNA scores of inferred miRNAs derived from different regions at the post-injury days 2, 5, and 7. c Gene expression of inferred miRNAs in the uninjured and injured regions with the scRNA-seq data. Significant differences between the two groups were calculated with the one-sided (less) Wilcoxon test. d Gene expression of inferred miRNAs in the uninjured and injured muscles with the bulk miRNA-seq data. e Gene expression of Mir142a and its target gene Nmrk2 among the skeletal muscles at different time points. f Comparison of gene expression of Mir142a and its target gene Nmrk2 among the different regions of skeletal muscles at the post-injury day 2. Significant differences between the two groups for the miRNA gene were calculated with the one-sided (less) Wilcoxon test. One-sided (greater) Wilcoxon test for the target gene. g Functional annotations of miR- 142a- 5p and its target gene Nmrk2. h Gene expression of Mir145a and its target gene Ms4a6c among the skeletal muscles at different time points. i Comparison of gene expression of Mir145a and its target gene Ms4a6c among the different regions of skeletal muscles at the post-injury day 2. j Functional annotations of miR- 145a- 5p and its target gene Ms4a6c. Significant differences between the two groups for the miRNA gene were calculated with the one-sided (less) Wilcoxon test. One-sided (greater) Wilcoxon test for the target gene. k Correlation between the scaled module scores of the skeletal muscle tissue development and skeletal muscle cell different signatures and the scaled expression of the target gene Ms4a6c of miR- 145a- 5p. Spearman coefficient (rho) was used. The bar plot showed the mean ± SEM. The number of data points in the uninjured group involving the myofiber is 1045. The number of data points in day 2 groups involving the myofiber, injury border, and injury locus are 272, 197, and 561, respectively