Fig. 7

Identification of the granulocyte-hepatocyte communication discriminating between normal and fatty liver transplantation. a The rat scRNA-seq dataset of the normal and high-fat diet transplanted livers including granulocytes, MPs, monocytes, DCs, hepatocytes, NK cells, Kupffer cells, T cells, Endo, and B cells. b EV-derived MiTI-mediated CCC inferred by miRTalk, displaying the number of MiTIs between pairwise cell types in normal and high-fat diet livers. c Heatmap showing the miRNA scores of inferred miRNAs derived from different senders. The receivers include B cells, DCs, endos, hepatocytes, Kupffer cells, T cells, monocytes, and NK cells in normal and fatty livers and granulocytes in normal liver. d Gene expression of inferred miRNAs in the sender cell types of normal and fatty transplanted livers with the scRNA-seq data. The numbers of data points in normal and high-fat groups involving the granulocyte are 3874 and 4245, respectively. The numbers of data points in normal and high-fat groups involving the hepatocyte are 1736 and 2449, respectively. Significant differences between the two groups for Mir223 and Mir290 were calculated with the one-sided (greater) Wilcoxon test. One-sided (less) Wilcoxon test for Mir21. (e) Fold change (FC) of gene expression of inferred miRNAs in the liver under the conditions of pre-LT and post-LT. f Specificity for all miRNAs in senders regarding target genes in hepatocytes of high-fat transplanted livers. g Functional annotation of hsa-miR- 223 - 3p and its target gene Vim. h Expression of Col1a1 and module score of the TGF-beta pathway signatures in hepatocytes of normal and high-fat transplanted livers. Significant differences between the two groups were calculated with the one-sided (less) Wilcoxon test. i Gene set enrichment analysis (GSEA) with gene sets from MSigDB by comparing the differentially expressed genes (DEGs) of hepatocytes of normal and fatty transplanted livers. Significant differences between the two groups were calculated with the one-sided (less) Wilcoxon test. j Expression of Mir223 and its target gene Vim among hepatic non-parenchymal cells of transplanted livers. k Functional annotation of hsa-miR- 223 - 3p and its target gene Vim. l Expression of Vim and module score of apoptosis in hepatocytes of normal and high-fat transplanted livers. The number of data points in normal and high-fat groups involving the hepatocyte are 865 and 355, respectively. m The survival rate of BRL- 3 A after OGD/R treatment and expression of Vim evaluated by RT‐qPCR. The number of data points in each group is 3. Significant differences between the two groups for the survival rate were calculated with the one-sided (less) T-test. One-sided (greater) T-test for the Vim level. n Predicted duplex structures for rno-miR- 223 - 3p and its target gene Vim by miRanda. MP, macrophage; DC, dendritic cell; NK, natural killer; Endo, endothelial cell. LT, liver transplantation. OGD/R, oxygen–glucose deprivation/reperfusion. The bar plot showed the mean ± SEM