Fig. 2

A Estimated proportion of genes with region-specific library size effects. On average, CosMx and STOmics datasets have the highest proportion of genes exhibiting region-specific effects, followed by Xenium. Visium datasets have the lowest proportion. B Adjusted Rand Index of clusters identified using differently normalized data vs annotated spatial regions. Boxplots show the summary by platform. The colored bars above each group of boxplots indicate the best-performing method for each dataset that makes up the group, based on maximum (darker-shade) and median (lighter-shade) statistics