Fig. 2

Transcriptome-wide association studies of fatty acids. a Venn diagram shows TWAS significant genes for FAs at 20 and 40 days after flowering (DAF). b An upset plot displays TWAS significant gene sets associated with the FAs. The bar on the left represents the original number of the significant genes for seven FAs, while the bar above shows the number of intersecting genes. Circles and connecting lines below provide the phenotypic information for calculating the intersecting genes. c Manhattan plot of C18:1 at 20 DAF. d Manhattan plot of C18:1 at 40 DAF. Each point represents a gene, with those positively or negatively associated with C18:1 depicted above or below the thick black line, respectively. Gold dots indicate significant lipid metabolism genes previously identified in Arabidopsis. e Venn diagram shows the significant C18:1 associated genes from TWAS at 20 and 40 DAF. f GO enrichment analysis of significant overlapping C18:1 associated genes at 20 and 40 DAF. The y-axis represents the names of the enriched pathways. The count value indicates the number of overlapped genes in each pathway, while the FDR value reflects the significance of enrichment