Fig. 6

Hmgb2-dependent promoters contain an E-box and Pou2f2 factor binding motif a Venn diagram illustrating the overlap in ATAC signals for MASs after Neurog2 overexpression in EGF + bFGF and bFGF cultures, with MASs induced by Neurog2 and Hmgb2 overexpression in bFGF culture 48 h after viral transduction. b, c Plots depicting enriched GO biological process terms in 395 peak set MASs in panel a (b) and 268 peak set MASs in panel a (c). Red text represents the GO terms associated with neuronal fate. d, e Transcription factor consensus sequences identified in 268 peak set MASs in panel a (d) and 395 peak set MASs in panel a (e), identified with de novo motif analysis. The motif image from the BaMM web server shows the likelihood of each nucleotide at each motif position. The color intensity reflects the probability, with darker colors indicating higher probabilities. Tables show transcription factors binding these motifs. f, g Dot plots show the enrichment of selected promoters from genes associated with the GO term “modulation of chemical synaptic transmission” (Fig. 6b), compared to 10% input, using the FLAG antibody (targeting Neurog2-FLAG) and the Hmgb2 antibody in bFGF culture. Data are shown as median ± IQR; single dots represent independent biological replicates. Significance was tested with two-tailed Mann-Whitney test. Abbreviations: MAS, more accessible site; TF, transcription factor