Fig. 5

Hmgb2 improves the capability of Neurog2 to open promoters of neuronal maturation-associated genes a,b Heat maps depicting opening of promoters by Neurog2 and Hmgb2 or their combination in EGF + bFGF (green, a) and bFGF (magenta, b) culture. Scale: 1 kb (c) Heat maps depicting ATAC signals in the promoters of the core neurogenic genes (Fig. 4d) 48 h after Neurog2, Hmgb2, or Neurog2 + Hmgb2 overexpression in EGF + bFGF and bFGF cultures. d IGV tracks showing the ATAC signal in the promoters of genes identifying non-neuronal lineages 48 h after Neurog2, Hmgb2, or Neurog2 + Hmgb2 overexpression in bFGF culture. e Histogram depicting the number of more (MAS) or less (LAS) accessible sites identified by ATAC 48 h after Neurog2, Hmgb2, or Neurog2 + Hmgb2 overexpression in EGF + bFGF (green) and bFGF (magenta) cultures. f Plot depicting enriched GO biological process terms in the promoter set opened by Neurog2 + Hmgb2 in bFGF culture 48 h after viral transduction. Red text represents the GO terms associated with neuronal fate. g Heat map showing ATAC signal in the promoters of neuronal maturation-related genes (red in panel f) 48 h after Neurog2, Hmgb2, or Neurog2 + Hmgb2 overexpression in bFGF culture. g IGV tracks showing the ATAC signal in the promoters of representative genes involved in neuronal maturation 48 h after Neurog2, Hmgb2, or Neurog2 + Hmgb2 overexpression in FGF culture. Green boxes indicate differentially accessible sites