Fig. 4

Neurog2 induces incomplete neuronal fate in bFGF culture a, b Plots depicting enriched GO biological process terms in gene sets induced by Neurog2 in EGF + bFGF culture (a) and bFGF culture (b) 48 h after viral transduction. Orange text represents the GO terms not associated with neuronal fate. Green and magenta text represent GO terms specifically enriched in EGF + bFGF culture and bFGF culture, respectively. c Venn diagram illustrating the overlap of Neurog2-induced transcripts in EGF + bFGF and bFGF culture 48 h after viral transduction. d Heat map showing Neurog2 − or Neurog2 + HMGB2-mediated induction of core neurogenic factors (according to Masserdotti et al., 2013) in EGF + bFGF and bFGF cultures. e Dot plot depicting the proportion of transduced cells converting to neurons in EGF + bFGF and bFGF cultures 7 days after transduction in Prox1 deficient or Prox-1 overexpressing cells. Data are shown as median ± IQR; single dots represent independent biological replicates. Significance was tested with two-tailed Mann-Whitney test. f Plot showing GO terms enriched in the gene set upregulated in bFGF culture by Neurog2 and Hmgb2 expression 48 h after viral transduction. GO terms in green text are also induced by Neurog2 alone in EGF + bFGF culture (panel a). Orange text represents the GO terms not associated with neuronal fate. g Venn diagram illustrating the overlap of Neurog2-induced transcripts in EGF + bFGF and bFGF culture with Neurog2 and Hmgb2-induced transcripts after overexpression in bFGF culture 48 h after viral transduction. h Plot depicting enriched GO biological process terms in gene sets induced in the reprogramming prone condition (46 genes set; Fig. 4g). GO terms in green text are also induced by Neurog2 alone in EGF + bFGF culture. Orange text represents the GO terms not associated with neuronal fate. Abbreviations: FP, fluorescent protein; GO, Gene Ontology