Fig. 2

Hmgb2 is sufficient for successful Neurog2-mediated direct astrocyte to neuron conversion a Scheme depicting the experimental paradigm used for astrocyte to neuron conversion. b,c”’) Micrographs showing the identity of Neurog2- and Hmgb2-expressing virally transduced cells 7 days after transduction in EGF + bFGF (a) and bFGF cultures (b). b’, b”, b”’, c’, c”, and c”’ are magnifications of the boxed areas in a and b, respectively. Yellow arrows indicate co-transduced cells expressing Neurog2 and Hmgb2, yellow arrowheads indicate cells transduced only with Hmgb2-encoding virus, and blue arrowheads indicate cells transduced with only Neurog2-encoding virus. Scale bars: 100 µm in b and c; 50 µm in b’. b”, b”’, c’, c”, and cb”’. d Dot plot depicting the proportion of transduced cells converting to neurons in EGF + bFGF and bFGF cultures 7 days after transduction. Data are shown as median ± IQR; single dots represent independent biological replicates. Significance was tested with two-tailed Mann-Whitney test. e Histogram depicting the identities of cells transduced with the indicated factors 7 days after transduction. Abbreviation: FP, fluorescent protein