Fig. 4

Tissue specific TFs are regulated at transcription. Ubiquitous factors are post-transcripionally regulated. A Histogram of the transcription level (x-axis) of a tissue specific TF (GRHL2; purple) and ubiquitous TF (CREB1; pink) across the nascent RNA-seq datasets. B Cumulative distribution function of the transcription of the gene encoding the TF (RPKM) for a set of high confidence tissue specific factors (purple) and ubiquitous factors (pink) across 126 control experiments. The relationship between the transcription level of the gene encoding the TF (x-axis) and observed MD-score (y-axis) for (C) ubiquitous TF (KLF12) and (D) a tissue specific TF (Nanog). HOCOMOCO PSSMs shown in lower right corner. E Plot of the significance of the MD-score (top) and the transcription of the gene encoding the TF (bottom) for all TFs (gray), highlighting KLF12 (left, pink) and Nanog (right, purple). F Violin plots of frequency of expression in single cell RNA-seq [63] across 172 tissues for ubiquitous (pink), tissue specific (purple), and all TFs (gray). G Violin plots of frequency of expression in single cell RNA-seq [63] across 172 tissues for TFs with the bottom 10% (green) and top 10% (gold) GC content within their PSSMs. H Histogram of the number of tissues that a tissue specific TF (GRHL2; purple) and ubiquitous TF (CREB1; pink) are expressed in by single cell RNA-seq [63]. I Cumulative distribution function of the steady-state RNA level (scRNA-seq) for the same high confidence tissue specific factors (purple) and ubiquitous factors (pink) across 172 tissues from atlas of fetal gene expression [63]