Fig. 7

CLCN2 expression in the embryonic skin. A Cell-type assignment of the nine clusters we identified in the embryonic skin of a wild-type corn snake based on single-cell transcriptomic analyses. Uniform manifold approximation and projection (UMAP) representation of (B) the overall expression level of CLCN2 and (C) its expression within the chromatophores cluster. D Whole mount in situ hybridization of wild-type embryos with a species-specific CLCN2 probe. Dorsal view of the anterior part of the embryos at developmental stages 4, 5, 6, and 7. CLCN2 expression is barely detectable. Inset for S7: magnification of the region in the dashed rectangle. E WISH of Motley and Stripe embryos with a PMEL probe. At stages 4 and 5, we observe uniform expression. At stage 8, the pattern is already established with fused blotches and stripes visible on the Motley and Stripe embryos, respectively. Scale bars: 1 mm