Fig. 3
From: Systematic interrogation of functional genes underlying cholesterol and lipid homeostasis
Identification of potential cholesterol regulators at the transcriptional level. A Venn diagram showing the overlap of up- or down-regulated DEGs of HepG2 cells upon LDLR KO, HMGCR, or double KO (DKO) determined by RNA-seq analysis. B Heatmap showing the DEGs across different samples of HepG2 cells. C Volcano plot showing the DEGs in DKO cells compared to control HepG2 cells with several typical genes highlighted. D The top five enriched functional terms for either up-regulated or down-regulated DEGs in DKO cells compared to control HepG2 cells by GO and KEGG analysis. E Venn diagram showing the overlap of DEGs between sterol deprivation condition (sterol depleted vs. normal) and SREBF2 KO under sterol deprivation (SREBF2 KO vs. vector control) in HepG2 cells. F Heatmap showing the DEGs across indicated samples of HepG2 cells. G Volcano plot showing the DEGs between sterol deprivation and normal conditions of HepG2 cells with several representative genes highlighted. H Volcano plot showing the DEGs in SREBF2 KO cells compared to vector control HepG2 cells in sterol deprivation condition with several representative genes highlighted. I Venn diagram showing the overlap of DEGs for indicated comparison groups. J Heatmap showing the expression change of representative DEGs that were shared between at least three comparison groups of I