Fig. 8

Complementation with CBP restores DNA damage repair in irradiated-CBP knockout T47D cells. a, b Upper panel: T47D CBP knockout (KO) cells were transfected with the indicated plasmids and irradiated with 2 Gy. Immunofluorescence analysis was performed for γH2AX (red) (a), 53BP1(green), and Flag (red) (b) after 24 h of ionizing radiation (IR). Scale bar, 100 µm. Lower panel: Quantification of γH2AX foci (red) per cell was done in green fluorescence protein (GFP)-positive cells versus GFP-negative cells for a. Quantification of 53BP1 (green) foci per cell was done in Flag-positive cells (red) versus Flag-negative cells for b. Data are represented as mean ± SEM from 20 cells, n = 3. P < 0.05 is considered significant, Mann–Whitney U test