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Fig. 2 | Genome Biology

Fig. 2

From: The histone acetyltransferase CBP participates in regulating the DNA damage response through ATM after double-strand breaks

Fig. 2

CBP depletion impairs DNA double-strand breaks repair in MCF7 and T47D cells. a Left panel: Immunofluorescence of γH2AX (green), merged with DAPI staining (blue) of nuclei, after 8 h treatment with Doxorubicin (DOX) in MCF7 and T47D cells transiently transfected with negative control (siCtrl) or CBP siRNA. Scale bar, 100 µm. Right panel: Average number of γH2AX foci per cell from 100 cells. b Left panel: Comet assay images showing DNA double-strand breaks repair kinetics following DOX removal in T47D cells transfected with the indicated siRNAs. Scale bar, 50 µm. Right panel: Quantification of the neutral comet assay by tail moment at 0, 6, and 12 h post-DOX incubation in T47D cells, from 50 cells. c Colony formation assay in T47D cells transfected with siCtrl or siCBP for 48 h and treated with the indicated concentrations of DOX. Data are represented as mean ± SEM, n = 3. P < 0.05 is considered significant, Mann–Whitney U test

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Genome Biology

ISSN: 1474-760X

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