Fig. 6

Coordinated expression of ASPM and FOXM1 in HCC. A Protein levels of FOXM1 and ASPM in HCC samples were analyzed by IHC. The scale bar is 50 μm. B Protein levels (H-Score) of FOXM1 and ASPM in tumor and adjacent normal tissues were analyzed by IHC. The significance was analyzed by Student’s t test. **p < 0.01, ****p < 0.0001. C Analysis of the mRNA levels of ASPM and FOXM1 (cancer vs. normal) in multiple solid cancers from Oncomine database. D RNA levels of ASPM and FOXM1 in GEO datasets (GSE6764, GSE14520) and TCGA dataset of HCC. The data were presented as the mean ± SD of different samples. The significance was analyzed by Student’s t test. ***p < 0.001, ****p < 0.0001. E The correlation of FOXM1 and ASPM expression in 31 solid tumors (including ACC, BLCA, BRCA, CESC, CHOL, COAD, ESCA, GBM, HNSC, KICH, KIRC, KIRP, LGG, LIHC, LUAD, LUSC, MESO, OV, PAAD, PCPG, PRAD, READ, SARC, SKCM, STAD, TGCT, THCA, THYM, UCEC, UCS, and UVM) from the TCGA database was analyzed using the GEPIA platform (left), and the correlation of FOXM1 and ASPM expression in LIHC (n = 374) TCGA data (right). F The correlation between the mRNA levels of ASPM and FOXM1 in six HCC cell lines. mRNA expression was evaluated by qRT-PCR