Fig. 2

Multi-locus targeted ssuh2 gene mutation causes excessive skeletal muscle fibers growth in zebrafish. A Histological images of skeletal muscle of adult zebrafish obtained by hematoxylin and eosin staining. Wild type (WT) and ssuh2 F0 KO genotypes (left and right panels), respectively. B Muscle fiber area and perimeter measured in WT and ssuh2 F0 with n = 3 animals per genotype at 2.5 months post fertilization. Each dot on the graph corresponds to the muscle fiber area (491 vs 502 fibers per genotype) and perimeter (493 vs 501 fibers per genotype). Statistical analysis performed using Mann–Whitney test, ****p = 0.0001. Scale bar: 20 µM