Fig. 1

Silencing of CG4984 in Drosophila muscles led to functional decline and myofibrillar defect. A–C Mef2 > CG4984-IR (Mef2-Gal4:UAS-CG4984-RNAi) flies compared to control (Mef2-Gal4) flies. Mef2, myocyte enhancer factor 2. A Illustration of the concentric circles on the Petri dish that form the zones to determine locomotion. Graph displays the locomotion data based on the number of larvae that crossed each zone within a 1-min time interval. Each data point represents one larva. For each genotype, data are shown for three experiments of 10 larvae each (n = 30 larvae in total). Error bars correspond to SD. ** denotes significance of p < 0.01. B The vials show the difference in pupation height between the control and CG4984-RNAi flies. Quantitation in the bar graph shows the average pupation height in mm. Error bars correspond to SD. **p < 0.01. C Adult mobility measured by negative geotaxis. Three groups of ten female adults from each genotype were tested. The flies were tapped to the bottom. After 10 s, the number of flies above the 8 cm line was recorded. Averaged data are represented by percentages where the number of flies above the 8 cm mark is divided by the total number of flies tested within each group. Error bars correspond to SD. **p < 0.01