Fig. 2

Chimeric eCLIP of core C/D snoRNP proteins comprehensively recovers known C/D snoRNA interactions. A Schematic of chimeric eCLIP procedure. B Correlation of non-chimeric versus chimeric snoRNA read abundance from FBL chimeric eCLIP in mouse embryonic stem cells (mESC). Pearson correlation (R) is indicated. C Correlation of snoRNA chimeric read abundance from FBL versus NOP56 chimeric eCLIP in mESC. Pearson correlation (R) is indicated. D Chimeric read abundance for individual snoRNAs colored according to snoRNA functional class. The y-axis indicates average of chimeric RPM in FBL and NOP56 chimeric eCLIP. E Pie chart of total number of snoRNA chimeric reads from mESC according to snoRNA functional class from FBL chimeric eCLIP. F Browser tracks of non-chimeric reads and snoRNA chimeric reads mapped to pre-rRNA as captured by FBL and NOP56 chimeric eCLIP in mESC. Annotated Nm sites are shown. G Heatmap of chimeric read coverage across pre-rRNA and snRNA from FBL and NOP56 chimeric eCLIP for canonical snoRNAs. Known Nm target sites are shown in black. H Metagene plots of mESC chimeric read coverage flanking Nm (red) and pseudouridine (black) sites in rRNA and snRNA. Chimeric RPM, reads per million chimeric reads. Data shown is from 1 replicate per RBP