Fig. 2

Alignment and prediction of single-cell profiles from mouse to human in primary motor cortex. A UMAP of cells across species, colored by species. B UMAP of cell embeddings learned in Icebear, where cell embeddings are independent of species factor. C Barplot showing Pearson correlation coefficient (cor) between predicted and observed gene expression profiles in the “held-out” major cell types in human motor cortex. For each cell type, the Pearson correlation coefficient is compared between Icebear’s prediction (dark green bar) and two baselines (species and cell type). We also compared “indiv_donorbaseline” (correlation between individual donors) with Icebear’s correlation with individual donors (“indiv_prediction”, light green bar). D Similar to C, we evaluated Icebear’s performance on predicting high-resolution cell type specific profiles. E Boxplots of genes absolute log2FC between predicted and true human profiles per cell type (grouped by whether they are housekeeping genes or not). F Boxplots of genes relative prediction error across cell types