Fig. 3

CENP-B binding in horse, Grevy’s zebra, and mule. A Localization of CENP-B protein and CENPB-sat on horse and Grevy’s zebra metaphase chromosomes by immuno-FISH. Left: CENP-B signals (green) on DAPI-stained chromosomes (blue). Middle: FISH CENPB-sat signals (red) on the same metaphase spreads. Immunofluorescence and FISH signals were acquired separately. White arrows point to examples of chromosomes with CENPB-sat but without CENP-B signals. Right: DAPI staining of the same chromosomes. B A schematic representation of the CENPB-eGFP construct used in transfection is shown on top. Detection of eGFP tagged CENP-B (green) in three horse metaphase spreads. A CENP-B positive and two CENP-B negative chromosomes are boxed and zoomed in the top panel. C Localization of CENP-A and CENP-B in mule primary fibroblasts. Immunofluorescence with an anti-CENP-A serum (green) and an anti-CENP-B antibody (red) on DAPI-stained metaphase chromosomes