Fig. 2

Localization of CENP-B and CENPB-sat in the four species. First column: double immunofluorescence with an anti-CENP-B antibody (red) and an anti-CENP-A serum (green) on DAPI-stained metaphase chromosomes (blue). Second column: double immunofluorescence with an anti-CENP-B antibody (red) and an anti-CENP-C serum (green) on DAPI-stained metaphase chromosomes. Third column: FISH localization of CENPB-sat (red) on DAPI-stained metaphase chromosomes. Fourth column: schematic representation of CENP-B and CENPB-sat signals on metaphase chromosomes. Loci hybridizing with the CENPB-sat probe only are labeled in red. Loci hybridizing with the CENPB-sat probe and positive to CENP-B immunofluorescence are labeled in yellow