Fig. 5
From: Genome-wide CRISPR guide RNA design and specificity analysis with GuideScan2
GuideScan2 enables design of a new gRNA library for allele-specific editing in a hybrid genome. a Experimental validation of selected C57BL/6- and 129S1/SvlmJ-specific gRNAs in mES cells of the F1 C57BL/6 \(\times\) 129S1/SvlmJ hybrid mice. Asterisk marks manually chosen gRNAs, other gRNAs were chosen at random from high-confidence allele-specific gRNAs (“Methods ” section). b Total number of allele-specific gRNAs by category genome-wide (in millions). (B6, C57BL/6; 129S1, 129S1/SvlmJ)