Fig. 3
From: Genome-wide CRISPR guide RNA design and specificity analysis with GuideScan2
GuideScan2 reveals widespread confounding effects of low-specificity gRNAs in published CRISPR screens. a Reanalysis of published essentiality CRISPRko screens for non-essential genes (smoothed conditional means, “Methods” section). b Reanalysis of published CRISPRi screens. Comparison of gene-level specificity (mean over gRNAs per gene) for genes identified by the authors as hits and non-hits. Empirical cumulative distribution function; Kolmogorov–Smirnov test, two-sided