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Fig. 1 | Genome Biology

Fig. 1

From: Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells

Fig. 1

SsdAtox-CBEs exhibit mutagenic activity. a Phylogenetic tree analysis of ssDNA and dsDNA cytidine deaminases used in cytosine base editors (CBEs). b Architecture of the SsdAtox CBE system. Pro: promoter, NLS: nuclear localization sequence, nCas9: SpCas9 D10A nickase, UGI: uracil glycosylase inhibitor, Ter: terminator. c Viability in colony-forming units of A. tumefaciens GV3101 strains after transformation of binary vectors containing SsdAtox-CBE or hA3A-CBE. d Viability in colony-forming units of E. coli Top10 strains after transformation of binary vectors containing SsdAtox-CBE or hA3A-CBE. Values and error bars indicate the mean ± SEM, n = 3 independent experiments. ** P < 0.01; n.s. (not significant) using Student’s two-tailed unpaired t-test. e Sanger sequencing of SsdAtox coding region. Mismatches are highlighted in red and are indicated by red triangles. Encoded amino acids are indicated in single letter code above the chromatograms for the reference sequence and below the chromatograms for the mutant variant

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Genome Biology

ISSN: 1474-760X

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