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Fig.7 | Genome Biology

Fig.7

From: Transcription of a centromere-enriched retroelement and local retention of its RNA are significant features of the CENP-A chromatin landscape

Fig.7

lacO transcription is coupled with de novo centromere formation. A Sequential IF/RNA/DNA-FISH on larval brains from GFP-LacI and CAL1-GFP-LacI/3peri, both (lacO array at 3peri). IF for CENP-C is shown in green. RNA and DNA-FISH with a lacO probe are shown in yellow. DNA-FISH for dodeca is shown in cyan. B Bar graphs showing the frequency of lacO transcription in GFP-LacI and CAL1-GFP-LacI/3peri in metaphase and C in interphase (Fisher’s exact test, N = 5 brains, n = 15 spreads/brain). D Scatter plot showing the fluorescence intensity of lacO RNA-FISH in GFP-LacI and CAL1-GFP-LacI/3peri in metaphase (nested t-test, N = 3 brains, n = 6–11 spreads/brain). A.U. stands for arbitrary units

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Genome Biology

ISSN: 1474-760X

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