Fig. 7

ASH1L-mediated H3K36me2 deposition is targeted to regulatory elements of developmentally relevant genes. a Genome-browser tracks of MS-normalized H3K36me2 signal for QKO and QuiKO, depicting a region of punctate peaks remaining in QKO cells that are depleted upon subsequent loss of ASH1L. Zooming into one of the peaks shows that they are still quite broad (10–70 kb). Three replicates were merged (n = 3) for each ChIP-seq and ATAC-seq track. H3K36me2 ChIP-seq signals were MS normalized and represent mean local frequency of the relevant modification. ATAC-seq signals were normalized by library depth. b Pie chart depicting the distribution of remaining H3K36me2 peaks in QKO cells. Overlapping peaks from three biological replicates were exclusively used in the analysis. c Enriched motifs found in open chromatin regions (as marked by ATAC-seq peaks) within H3K36me2 domains in QKO cells, indicating potential recruitment factors for ASH1L