Fig. 4

Intergenic H3K36me2 decreases following NSD1-KO and is further reduced in H3K36M-OE and multi-knockout conditions. a Genome-browser tracks of H3K36me2 signal centered on intergenic regions (n = 9551) flanked by genic regions, highlighting reduced intergenic signal following loss of NSD1, more pronounced reductions in NSD1/2-DKO and H3K36M-OE cells, and the most significant depletions in subsequent multi-knockout conditions. For each cell line, three replicates (n = 3) were merged, except for DKO and K36M-OE where two replicates were merged. ChIP-seq signals were MS normalized and represent mean local frequency of the relevant modification. b Heatmaps showing H3K36me2 (input- and depth-normalized only) enrichment patterns ± 20 kb flanking IGRs, summarizing the H3K36me2 trends for multiple cell lines at IGRs. c H3K36me2 “peakiness scores”, representing the average ChIP signal in the top 1% of 1-kb bins over the total signal, indicating more punctate distributions of H3K36me2 in multiple-KO conditions. Individual data points represent biological replicates for each condition (n = 3 per condition, except for DKO and K36M-OE where n = 2). Error bars represent mean ± standard deviation