Fig. 3

Effects of K36MT ablations and H3K36M-OE on H3K36me1 distribution patterns within genes. a Genome-browser tracks of MS-normalized H3K36me1 signal showing depletion in H3K36M-OE and multiple-KO conditions, and an increase in SETD2-KO cells. The two bottom tracks illustrate that despite NSD1/2/3-SETD2-QKO and NSD1/2/3-SETD2-ASH1L-QuiKO displaying reduced amounts of H3K36me1, the distribution patterns remain similar to that observed in parental cells. For each cell line, three replicates (n = 3) were merged. ChIP-seq signals were MS normalized and represent mean local frequency of the relevant modification. b Boxplots of H3K36me1 genic to intergenic ratios, indicating that the increase in SETD2-KO cells is found primarily within genic regions (n = 3 per condition). c Boxplots showing the exon versus intron H3K36me1 signal in parental and SETD2-KO cells, indicating increased signal at exons following SETD2-KO (n = 3 per condition). In the boxplots for b and c, boxes span the lower (first quartile) and upper quartiles (third quartile), median is indicated with a center line and whiskers extend to a maximum of 1.5 times the interquartile range