Fig. 6

Ptprc loss attenuates macrophage phagocytosis of malignant cells. A Schematic of time lapse microscopy assays for co-cultured J774 macrophages (Ptprc knockdown or control) and Ramos lymphoma cells. pHrodo red intracellular indicator dye is used to measure extent of phagocytosis following opsonization of malignant cells with α-CD47 and α-CD20 antibodies. B Distribution of mean Ptprc (CD45) expression measured by flow cytometry. n = 3 replicates. C Phagocytosis time lapse microscopy assay for uptake of Ramos cancer cells by J774 macrophages. p values, two-way ANOVA comparing each Ptprc perturbation to both negative control sgRNAs. n = 3 cell culture wells. C Images of J774 macrophages with indicated genotypes co-cultured with pHrodo red-labelled malignant Ramos cells after 4 h incubation. Scale bar, 100 μm. Representative of two independent experiments performed in biological triplicate wells