Fig. 1

CRISPRi screens identify oncogenic drivers and modifiers of radiotherapy response in GBM. A Schematic of in vitro screen workflow to nominate candidates for perturb-seq. B Kaplan–Meier survival curves of GL261 intracranial tumor models treated with either fractionated radiotherapy (2 Gy × 5 fractions) to the whole brain or no treatment. p value derived from log-rank test. C Volcano plot of log2 enrichment for the top 3 sgRNAs corresponding to each gene target in the growth (left) or radiation to growth ratio (right) phenotypes in GL261 cells. A discriminant threshold score of 7 was used for both screen analyses to nominate hit genes for in vivo perturb-seq. MW = Mann-Whitney U. D Gene ontology enrichment analysis for negative growth (left) and negative radiation to growth ratio (right) screen hits identified in C