Fig. 7

Functional validation of regenerative RICs. A Scheme of functional experiments. B Removal of the regenerative bud at 6 hpa and its phenotype (11 dpa), scoring, fibrosis (fibronectin staining, 8 hpa), and defective ROC migration (RT-qPCR of ROC markers 1 dpa). C Loss of function of three RIC markers: pmepa1, mmp8, and mmp9 using Vivo MO. D Phenotypes and scoring with extensive fibrosis (fibronectin IHC) and defective ROC migration (RT-qPCR for ROC markers). E Extensive fibrosis and defective ROC migration in refractory samples. Brightfield and confocal images scale size is 300 μm. RT-qPCR was prepared from at least biological triplicates, each containing at least five dissected regenerating tails. Significance tested with Student’s t-test (ns not significant, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Fibronectin staining is shown using one representative sample from at least seven biological replicates. Green positive sign indicates regenerative while red negative sign indicates refractory sample