Fig. 5
From: Characterization of regeneration initiating cells during Xenopus laevis tail regeneration
Spatial transcriptomics of tail regeneration. A Scheme of the spatial transcriptomics experiment. B Clusters formed based on enriched expression in regenerative and refractory samples. Ten clusters in the Loupe software were manually annotated based on top cluster markers and comparison with scRNA-Seq data. C Volcano plot showing differential expression between regenerative bud and the remaining spots. Bar plot shows the top 10 enriched GO terms associated with the overexpressed genes. D Comparison of spatial expression of selected RIC markers in regenerative and refractory samples at 1 dpa. E Distribution of RICs in regenerative and refractory samples at 1 dpa based on deconvolution using scRNA-Seq data. F ROC and myeloid markers (based on scRNA-Seq) in regenerative and refractory samples at 3 dpa. Green positive and red negative signs indicate regenerative and refractory sample, respectively. Sections are shown from anterior (left) to posterior (right) of the tail bud region