Fig. 4

Comparison of score distributions for Splam (green) and SpliceAI (orange) when applied to 25,000 randomly chosen splice sites from chimpanzee (left), mouse (center), and Arabidopsis thaliana (right). Results for donor sites are shown across the top, and acceptor sites on the bottom. Scores assigned by each program are plotted along the x-axis, while densities for both donor and acceptor sites are plotted on the y-axis. The darkened vertical line through the distribution marks the median value, and the two neighboring dotted lines represent the first and third quartiles. The scores for Splam exhibit a narrow distribution that peaks near 1.0, illustrating that the vast majority of annotated splice sites are assigned high scores, even in the A. thaliana genome. Scores for SpliceAI are more spread out for the chimpanzee and mouse, and the scores for A. thaliana splice sites show an M-shaped distribution peaking at 0 and 1 with a median near 0.5, rather than just peaking near 1