Fig. 6
From: The GC-content at the 5′ ends of human protein-coding genes is undergoing mutational decay
Net change of GC substitutions along the ORF. Average number of the net change of GC substitutions along the ORF normalized to 40 bins were plotted for human (A), chimpanzee (B), mouse (C), rat (D), dog (E), and fox (F), with a sliding window of 100 bp. Average number of the net change of GC substitutions along the ORF at 4-fold degeneracy positions (GC4) normalized to 40 bins were plotted for human (G), chimpanzee (H), mouse (I), rat (J), dog (K), and fox (L), with a sliding window of 100 bp. Only genes with the ORF starting in exon 1 and the start codon (ATG) were included in the analysis. p values are from permutation tests of changes in GC-content between the beginning (bin 1) of the ORF compared to the middle (bin 20) of the ORF (see “Methods”)