Fig. 1

Schematic representation of three key concepts: technical replicates, methods reproducibility, and genomic reproducibility. The same sample is processed (library preparation) and sequenced multiple times, possibly in different laboratories, but using the same experimental protocols and sequencing platform. The output of these sequencing runs are technical replicates represented as FASTQ files. Data analysis is performed for each technical replicate multiple times to assess consistency of genomic results, which refers to methods reproducibility. Genomic reproducibility, on the other hand, evaluates the consistency of genomic results across technical replicates