Detecting haplotype-specific transcript variation in long reads with FLAIR2

Table 1 R2C2 nanopore sequencing numbers

	Pool 1	Pool 2	Pool 3
Total GB basecalled reads	18.5	7.10	9.56
Number basecalled reads	1,423,603	713,990	778,145
Total GB consensus reads	0.999	0.600	1.03
Median length of consensus reads	1046	1192	1816
Median accuracy (%)	99.2	98.7	99.0
Number aligned CTRL KD consensus reads	445,285	267,746	252,193
Number aligned ADAR KD consensus reads	379,472	184,312	169,506
Number aligned CTRL KD size-selected reads	-	-	6716
Number aligned ADAR KD size-selected reads	-	-	141,754

For each ADAR KD and control KD sample pool that was sequenced on a MinION, we report the total number of reads obtained from sequencing after basecalling, consensus calling, and minimap2 alignment to the hg38 genome. We also show the number of gigabases of reads after basecalling and consensus calling, as well as the median length of the consensus reads. We calculated an accuracy for each read and report the median for each sequencing run

ISSN: 1474-760X