Fig. 2

Identification of downregulated inosines with short- and long-read RNA-Seq. a Experimental workflow of ADAR knockdown in H1975 cells. b Western blot validation of ADAR knockdown. c Volcano plot of differentially expressed genes identified from Illumina sequencing. Red: genes with increased expression after ADAR knockdown; blue: genes whose expression went down; black: no change in expression. d Venn diagram comparison of the inosines with significant differences in expression identified with Illumina, R2C2 nanopore, or present in the REDIportal database (hg38 liftover). e IGV browser view of a downregulated inosine at chr14:52775760 in GNPNAT1 in the R2C2 data