Fig.4

scHolography recapitulates the spatial organization of human skin. a Spatial feature plots of markers for four major cell types of human foreskin ST data (KRT10, suprabasal cell; KRT5, basal cell; COL1A2, dermal cell; ACTA2, smooth muscle cell). b UMAP plot of human foreskin scRNA-seq data. c 3D visualization of all cell types in scHolography human foreskin reconstruction. d 3D visualization of four major cell types in scHolography human foreskin reconstruction. e scHolography 3D feature plot of marker genes for 4 major cell. f SMN distances between 4 major foreskin cell types and smooth muscle cells (suprabasal cells n = 1118; basal cells n = 804; dermal cells n = 1529; smooth muscle cells n = 119). Boxplots show the median with interquartile ranges (IQRs) and whiskers extend to 1.5 × IQR from the box. Two-sided Wilcoxon tests are performed. g scHolography 3D plot of Cell #88 and its first-degree neighbors. h SMN cell type composition plot of each human foreskin cell type