Fig. 4
The m6A identification for A. thaliana and human using DENA. a Venn diagram shows the common m6A sites identified by DENA among Col-0, VIRc and vir-1 samples. b Jointplot shows the correlation of m6A rates at 28299 overlapped sites between Col-0 and vir-1. c Jointplot shows the correlation of m6A modification rate at 35,048 overlapped sites between Col-0 and VIRc. d Venn diagram shows the common m6A sites identified by DENA from wild-type (WT) and METTL3-knockout (M3KO) human cells. e The m6A distribution on transcripts in WT and M3KO, respectively. f Jointplot shows the correlation of m6A rates at 7036 overlapped sites between WT and M3KO. g Pie charts display the performance of DENA on 4201 miCLIP-identified m6A sites that are supported at least 50 reads, and 3768 sites out of them are regarded as m6A modification sites by DENA. h Flowcharts illustrating the identification of m6A sites using Bst 2.0 DNA polymerase Splint ligase with qPCR. Dp and Up are down probe and up probe, respectively. i The real-time fluorescence curves produced at Site_7340 and Site_7207 on the mRNA of PRP8A, respectively. A is Site_7207 and m6A is Site_7340 on PRP8A. j Bar plot demonstrates consistency of m6A rate at the four known modified sites between DENA detection and SCARLET identification, and the significant overestimation in the prediction from Nanom6A