Fig. 2

scRNA-seq analysis reveals lineage progenitors in EBs. a t-SNE plots of Primed H9 and EBs (day 4 EBs and day 8 EBs). We defined three progenitor clusters in day 4 EBs, including progenitor cell-2, progenitor cell-10, and progenitor cell-11. We defined six progenitor clusters in day 8 EBs, including muscle cell, liver cell, neural cell, stromal cell, epithelial cell, and endothelial cell. b Heatmap shows the expression pattern of top 15 differential genes in each progenitor cell. Differential genes of each cell type are listed in Additional file 4: Table S3. c Violin plots show the expression level distributions of marker genes across cell types. Cell types are represented by different colors in (a), (b), and (c)