Fig. 1

Pipeline used to obtain the A. filiformis developmental transcriptome. a Developmental timepoints used for RNA-seq: 9hpf corresponds to a late cleavage stage, 18hpf to a blastula stage, 27hpf to a mesenchyme blastula stage and 39hpf to a late gastrula stage (arrows point to position where spicules are formed). The brittle star A. filiformis and the sea urchin S. purpuratus pluteus larvae showing general morphological features and the birifrangent extended skeleton (m mouth, St stomach, Sk skeleton). b Assembly pipeline showing the individual steps and the reduction in sequences