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Fig. 2 | Genome Biology

Fig. 2

From: Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations

Fig. 2

Nonsense-mediated degradation of endogenous NMD substrates is inhibited by ASOs targeting NMD factors. Mouse MHT cells were treated with ASOs targeting mouse NMD factors by free uptake at the indicated concentrations. mRNA levels of the ASO-targeted NMD factors and  endogenous NMD substrates Atf3, Gadd45b, Upf1, Upf2,Upf3b, Smg1, and Smg5 were measured by qPCR analysis. Results are presented as means ± standard errors (n = 3). Mouse Gapdh mRNA was used as endogenous control. The mRNA level of each gene in untreated (NT) MHT cells was set as 1. a Upf1-ASO treated cells. b Upf2-ASO treated cells. c Smg1-ASO treated cells. d Smg6-ASO treated cells. e Upf3b-ASO treated cells. f Smg5-ASO treated cells. g Smg7-ASO treated cells. h Smg8-ASO treated cells. i Smg9-ASO treated cells. j Upf3a-ASO treated cells. Statistical significance was determined using a two-way ANOVA and Dunnett’s multiple comparison test in Prism. All groups were compared to NT group within each measurement. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

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Genome Biology

ISSN: 1474-760X

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