Fig. 6
From: The human lncRNA LINC-PINT inhibits tumor cell invasion through a highly conserved sequence element
PRC2 mediates LINC-PINT-dependent silencing of invasion genes. a Level of enrichment in SUZ12 immunoprecipitates of the indicated coding and non-coding RNAs in HCT116 cells. IgG is used as control. b EZH2 and SUZ12 proteins bound to LINC-PINT or antisense RNA (control RNA) when incubated with nuclear extracts. An unspecific cross-reacting protein is shown as control. c Expression changes of genes in LINC-PINT overexpressing HCT116 cells upon EZH2 depletion by shRNA. d, e SUZ12 (d) or H3K27me3 (e) enrichment in promoter regions of LINC-PINT-regulated genes in control or LINC-PINT HCT116 cells. Enrichment values are relative to the input. Mean ± SD of three qPCR replicates of a representative experiment are shown. f FA crosslinking and immunoprecipitation (fRIP) of SUZ12-bound LINC-PINT in HCT116. qRT-PCR identifies the LINC-PINT region bound by PRC2 in vivo. The scheme represents the location of the oligos along LINC-PINT transcript; E exon, I intron. g RNAs corresponding to FL or different fragments of LINC-PINT or its antisense sequence (AS-FL) were obtained by in vitro transcription. Their interaction with recombinant purified PRC2 was tested by RNA pull-down and SUZ12 and EZH2 was detected by western blot