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Fig. 7 | Genome Biology

Fig. 7

From: The developmental regulator PKL is required to maintain correct DNA methylation patterns at RNA-directed DNA methylation loci

Fig. 7

The correlation between PKL affected loci and repressive histone modifications. a H3K9me2 and H3K27me3 levels at the transgenic and endogenous RD29A (tRD29A and eRD29A) promoter measured by the chromatin immunoprecipitation (ChIP) assay. The ACT7 promoter (ACT7) serves as a negative control for the two repressive histone modifications. The ChIP DNA was quantified using real-time PCR and normalized to the signal at tRD29A in WT plants. Error bars represent standard deviations calculated from three biological replicates. b Distribution of nine different chromatin states on the whole genome or the CHH hypo-DMRs of the three mutants (nrpd1-3, nrpe1-11, and pkl-1). c, d Log transformed FDR values (–log10) of the overlap between hypoDMRs (c) and hyperDMRs (d) identified in pkl and the four repressive chromatin states

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Genome Biology

ISSN: 1474-760X

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