Fig. 4

Germ line transmission of founder alleles generated using Easi-CRISPR. a–f Genotyping of offspring from two founders each for the Pitx1 and Ambra1 conditional alleles (a, b) and one founder each of the Fgf8, Slc26a5, Mmp9, and Mmp13 knock-in alleles (c–f) showing germ line transmission from all of these founders. As expected, all the pups from the Fgf8 founder contain a targeted allele because the founder is biallelic (c)